Amplicon sequencing is frequently used to identify and differentiate microbial species. Short (<500 bp) hypervariable regions of conserved genes or intergenic regions are amplified by PCR and analyzed using NGS technology, and the resulting sequences are compared against microbial databases.
For bacteria and archaea, the 16S rRNA gene is the most common target for amplicon sequencing. For fungi, three targets are generally used: the 18S rRNA gene and two internal transcribed spacers (ITS) located between rRNA genes. These regions are usually sufficiently divergent to separate even highly related species, and can sometimes differentiate subspecies.
- 130-470 bp insert DNA library
- HiSeq platform, paired-end 250 bp
|TARGET||REGION||FRAGMENT LENGTH||PRIMER||PRIMER SEQUENCES (5’- 3’)|
* ITS1 is located between the 18S and 5.8S rRNA genes; ITS2 is located between the 5.8S and 28S rRNA genes.
Data Quality Guarantee
- The amount of data for each sample is not less than 30,000 tags, 50,000 tags or 100,000 tags.